What is PRP?

  • PRP is defined as a volume of autologous plasma that has a platelet concentration above baseline.
  • A blood sample typically contains 93% red blood cells, 6% platelets and 1% white blood cells. The rationale for PRP’s benefit lies in a reversed ratio of RBC to platelets, thereby increasing factors that would be more useful in healing. It is therefore believed that PRP can augment or stimulate healing by turning on the same biological healing process that normally occurs in the human body after injury.

Components of Red Blood Cells

  • Found in vasculature
  • Carry NO, CO2, O2
  • During oxidative stress, iron can release cytotoxic oxygen free radicals which can cause damage to DNA

Role in PRP: Complete elimination preferred

Components of White Blood Cells

  • Host defense agents (granulocytes)
  • Mediators of inflammatory response (monocytes in blood/macrophages in tissues, lymphocytes)

Role in PRP:

  • M1 (classic) – None (host defense)
  • M2 (alternate: IL-10) – Matrix deposition and remodeling
  • Lymphocytes release lymphokines which stimulate/inhibit fibroblast activity
  • Granulocytes regulate host defense – neutrophils release ROS which without target microbe can cause tissue damage – Removal from PRP ideal

Components of Plasma

  • Liquid component which suspends cells
  • Contains nutrients, hormones, vitamins, electrolytes, fibrinogen, albumin, and immunoglobulin

Role in PRP:

  • Nutrient rich for platelets and tissues alike
  • Fibrinogen converted to fibrin network which traps platelets at wound site to form a clot

Components of Platelets

  • Responsible for hemostasis
  • Construct new connective tissue
  • Promote vascularization

Role in PRP: Release of Growth Factors

EGF (Epithelial Growth Factor)- Promote epithelial cell growth, angiogenesis, and wound healing

VEGF (Vascular Endothelial Growth Factor)- Promotion of angiogenesis and would healing

IGF (Insulin Growth Factor)- Regulates Cell Growth

FGF (Fibroblast Growth Factor)- Tissue repair, cell growth, and collagen production

TGF-B (Transforming Growth Factor Beta)- Growth and Neogenesis of epithelial cell, vascular endothelial cell, and promotion of wound healing

PDGF (Platelet Derived Growth Factors)- Cell growth, new generation and repair of blood vessels, and collagen production

 

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About RegenKit

Platelet Rich Plasma - RegenKit Advantage

  • PRP, PRP-LR, PRP-LP, PRF options
  • Leukocyte Rich and Leukocyte Poor versions available
  • Highest PRP Yield per Blood Draw on the market
  • 8-10mls blood yields 4-5ccs PRP
  • Automatic Blood Draw
  • Automatic Platelet Separation
  • Adjustable concentration 1.7 to 5x over baseline
  • Over 1 Million Patients Treated World Wide
  • FDA Cleared and ISO 13485 certified
  • Class II Medical Device
  • Self Contained, Closed Loop
  • Conveniently packaged
  • As little as 5 minute processing time
  • Non-Pyrogenic
  • Patented Innovation
  • Consistant Cellular Isolation and Recovery
  • Over 80 Studies Indexed on Pub Med
  • Double Blister Sterilization for Safety
  • Up to 99.7% RBC removal (bct version)

The RegenKit Autologous Platelet-rich Plasma (A-PRP) system aids in the separation of the patient’s own blood components through the use of the RegenKit vacuum tube and clinical centrifuge. RegenKit  A-PRP is designed to deliver an increased concentration of bioactive factors and proteins through the concentration of platelets from whole blood. Once the blood is drawn into the RegenKit vacuum tube it is then spun under centrifugation according to centrifuge operating instructions. The PRP prepared using the RegenKit  is a platelet-rich plasma preparation with high platelet recovery and viability, a physiological level of leukocytes, and contains the entire plasma component of blood, which is rich in growth factors. The platelet–rich plasma is collected with syringes and can be mixed during application at the patient’s point of care.

Indications for Use

RegenKit is designed to be used for the safe a rapid perparation of autologous platelet rich-rich plasma (A-PRP) from a small sample of blood at the patient’s point of care. The PRP is mixed with autograft and/or allograft bone prior to application to an orthopaedic surgical site, as deemed necessary by clinical use requirements.

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